Ten (10) M Ammonium acetate is an efficient molecular concentration for the extraction of genomic DNA from small insects used for rolling circle amplification

Muturi, S. M.; Karanja, L. S.; Wachira, Francis N.; Wambulwa, Moses C.

dc.contributor.author	Muturi, S. M.
dc.contributor.author	Karanja, L. S.
dc.contributor.author	Wachira, Francis N.
dc.contributor.author	Wambulwa, Moses C.
dc.date.accessioned	2017-08-23T09:00:50Z
dc.date.available	2017-08-23T09:00:50Z
dc.date.issued	2013-09
dc.identifier.citation	African Journal of Biotechnology, Vol. 12(39), pp. 5790-5798, 25 September, 2013	en_US
dc.identifier.issn	1684-5315
dc.identifier.uri	https://www.researchgate.net/publication/263850157_Ten_10_M_Ammonium_acetate_is_an_efficient_molecular_concentration_for_the_extraction_of_genomic_DNA_from_small_insects_used_for_rolling_circle_amplification
dc.identifier.uri	http://repository.seku.ac.ke/handle/123456789/3515
dc.description	DOI: 10.5897/AJB2013.12848	en_US
dc.description.abstract	Most of the published methods for DNA isolation from vectors (insects) are not always effective in mealy-bugs, aphids and leafhoppers because they have high amount of polyphenols in their body systems. A simple, rapid, reliable labor-effective and carcinogenic compound-free salting out method for isolation of high molecular DNA from these insects is described. The ratio of OD260/OD280 ranged between 1.8 to 2.0. The method was successfully applied to Rolling Circle Amplification and in restriction analysis, indicating the removal of the common inhibitors.	en_US
dc.language.iso	en	en_US
dc.publisher	Academic Journals	en_US
dc.subject	Pseudococcidae	en_US
dc.subject	Aphididae	en_US
dc.subject	Auchenorryncha	en_US
dc.subject	DNA extraction	en_US
dc.subject	rolling circle amplification	en_US
dc.subject	restriction analysis	en_US
dc.title	Ten (10) M Ammonium acetate is an efficient molecular concentration for the extraction of genomic DNA from small insects used for rolling circle amplification	en_US
dc.type	Article	en_US